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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク History of electrophoresis ： ウィキペディア英語版 History of electrophoresis The history of electrophoresis begins in earnest with the work of Arne Tiselius in the 1931, and new separation processes and chemical analysis techniques based on electrophoresis continue to be developed into the 21st century. Tiselius, with support from the Rockefeller Foundation, developed the "Tiselius apparatus" for moving boundary electrophoresis, which was described in 1937 in the well-known paper "A New Apparatus for Electrophoretic Analysis of Colloidal Mixtures". The method spread slowly until the advent of effective zone electrophoresis methods in the 1940s and 1950s, which used filter paper or gels as supporting media. By the 1960s, increasingly sophisticated gel electrophoresis methods made it possible to separate biological molecules based on minute physical and chemical differences, helping to drive the rise of molecular biology. Gel electrophoresis and related techniques became the basis for a wide range of biochemical methods, such as protein fingerprinting, Southern blot and similar blotting procedures, DNA sequencing, and many more. ==Electrophoresis before Tiselius== Early work with the basic principle of electrophoresis dates to the early 19th century, based on Faraday's laws of electrolysis proposed in the late 18th century and other early electrochemistry. Experiments by Johann Wilhelm Hittorf, Walther Nernst, and Friedrich Kohlrausch to measure the properties and behavior of small ions moving through aqueous solutions under the influence of an electric field led to general mathematical descriptions of the electrochemistry of aqueous solutions. Kohlrausch created equations for varying concentrations of charged particles moving through solution, including sharp moving boundaries of migrating particles. By the beginning of the 20th century, electrochemists had found that such moving boundaries of charged particles could be created with U-shaped glass tubes.〔Vesterberg, pp. 4-5〕 Methods of optical detection of moving boundaries in liquids had been developed by August Toepler in the 1860s; Toepler measured the schlieren (''shadows'') or slight variations in optical properties that in inhomogeneous solutions. This method combined with the theoretical and experimental methods for creating and analysing charged moving boundaries would form the basis of Tiselius's moving boundary electrophoresis method.〔Vesterberg, p. 5〕 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「History of electrophoresis」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.